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猪DFAT细胞成脂再分化过程中KLF2和PPARγ的表达
猪 成熟脂肪细胞 去分化 再分化 KLF2 PPAR&gamma
2017/3/14
旨在探讨猪去分化脂肪细胞（DFAT）再分化过程中Krüppel样因子2（KLF2）和过氧化物酶体增殖物激活受体γ（PPARγ）的表达模式，为揭示猪DFAT细胞再分化机制奠定基础。采集猪成熟脂肪细胞，经“天花板”培养法获得DFAT细胞，用流式细胞仪检测表面抗原，形态学和油红O染色提取法检测成脂分化程度，Real-time PCR检测KLF2和PPARγ mRNA的表达。结果表明，猪成熟脂肪细胞接种第...
Differential Expression of PPARγ, FASN, and ACADM Genes in Various Adipose Tissues and Longissimus dorsi Muscle from Yanbian Yellow Cattle and Yan Yellow Cattle
PPARγ FASN ACADM Fat Deposition Cattle qRT-PCR
2016/5/16
The objective of this study was to investigate the correlation between cattle breeds and deposit of adipose tissues in different positions and the gene expressions of peroxisome proliferator-activated...