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Transgenic Expression of MsHsp23 Confers Enhanced Tolerance to Abiotic Stresses in Tall Fescue
Tall Fescue Forage Agrobacterium tumefaciens MsHsp23 Transformation
2016/5/10
Tall fescue (Festuca arundinacea Schreb.) is an important cool season forage plant that is not well suited to extreme heat, salts, or heavy metals. To develop transgenic tall fescue plants with enhanc...
Production of Transgenic Goats by Sperm-mediated Exogenous DNA Transfer Method
Sperm-mediated Gene Transfer Goat Sperm Cryopreservation Artificial Insemination (AI)
2016/5/3
In this study, the production of transgenic goats using sperm to integrate exogenous DNA and artificial insemination (AI) was carried out and the technical protocols for sperm-mediated gene transfer (...
GFP标记在转基因青鳉同系繁殖纯化中的应用Application of GFP Mark on Inbred Depuration of Transgenic Medaka (Oryzias latipes)
绿色荧光蛋白（GFP）基因 显微注射 青鳉 同系繁殖 纯化
2007/12/31
摘要从载体pBluescript SK+的Nde I位点处插入外源基因片段，构建了含有青鳉（Oryzias latipes）β-肌动蛋白（β-Actin）启动子和绿色荧光蛋白（GFP）标记基因的新表达载体。显微注射实验证实：在青鳉受精卵的单细胞期进行显微注射，标记基因的表达率和胚胎的存活率均较高。对嵌合体的转基因青鳉（F1）进行了两代的杂合体（F2和F3）筛选和一代的纯合体（F4）筛选，获得第4代...
精子介导转基因动物的制备Research on Spermatozoa-mediated Gene Transfer to Product Transgenic Animals
精子 脂质体 转基因动物
2007/12/30
摘要采用直接注射的方法，将脂质体包裹的含人乳铁蛋白基因的重组质粒pLNCXHLF注入兔睾丸组织和羊输精管中，一个月后分别与正常雌兔及正常的母羊交配。所产仔兔均为活体，经PCR和Southern检测,转基因仔兔阳性率平均为35%(11/31)；羔羊经引物F1/R1系统PCR检测阳性率为33.3％（4/12），F2/R2系统PCR检测阳性率为25%（3/12）。将流产羔羊组织解剖后提取舌、肺、肝脏、肌...
The Yield and Composition of Milk from Transgenic Rabbits
Rabbit Transgenic Milk Yield mWAP-hFVIII Gene
2016/4/25
Basic objective of this research was to compare the milk yield and composition of New Zealand White transgenic rabbit females expressing recombinant human factor VIII (hFVIII) in mammary gland during ...
Production of Transgenic Chimeric Chickens Using Blastodermal Cells
Chimeric Chickens Blastodermal Cells Transfection GFP
2016/4/18
A practical approach was proposed to produce transgenic chimeric chickens using blastodermal cells (BCs). The chicken BCs were mechanically dissociated and transferred into the recipient eggs that had...
Augmentation of Thermotolerance in Primary Skin Fibroblasts from a Transgenic Pig Overexpressing the Porcine HSP70.2
Heat Shock Protein 70.2 Transgenic Pig Thermotolerance
2016/4/18
A high environmental temperature affects the economic performance of pigs. Heat shock protein 70 (HSP70) has been reported to participate importantly in thermotolerance. This study aims to produce tra...
Generation of Embryonic Stem Cell-derived Transgenic Mice by Using Tetraploid Complementation
Human Resistin Tetraploid Embryonic Stem Cell Mouse
2016/4/18
The objective of this study was to generate transgenic mice expressing human resistin gene by using the tetraploid- embryonic stem (ES) cell complementation method. Human resistin gene was amplified f...
Cock Spermatozoa Serve as the Gene Vector for Generation of Transgenic Chicken (Gallus gallus)
Transgenic Chicken Sperm-mediated Gene Transfer
2016/4/15
To evaluate the feasibility of using sperm-mediated gene transfer (SMGT) for carrying foreign gene into chicken oocyte, a reporter gene, CX-EGFP, was used in this study. The reporter gene was first mi...
Expression of Human Serum Albumin in Milk of Transgenic Mice Using Goat β-casein/Human Serum Albumin Fusion Gene
Human Serum Albumin Transgenic Mouse Gene Expression
2016/4/15
The gene encoding human serum albumin (HSA) was cloned from human liver cDNA library by PCR. The HSA cDNA in size of 2,176 bp, including 1,830 bp of open reading frame, was cloned into the plasmid car...