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Disruptions to embryonic reprogramming alter adult mouse behavior
embryonic reprogramming adult mouse
2016/2/22
Right after fertilization, embryos at the earliest stages of development tell their genes: "Forget what it was like in the sperm or egg where you came from."
When the process of epigenetic reprogramm...
Sex Difference in Calbindin Cell Number in the Mouse Preoptic Area: Effects of Neonatal Estradiol and Bax Gene Deletion
Sex Difference Calbindin Cell Number Mouse Preoptic Area Neonatal Estradiol Bax Gene Deletion
2014/12/12
The sexually dimorphic nucleus of the preoptic area (SDN-POA) was first discovered in rats and is one of the most famous and best studied sex differences in the field of neuroscience. Though well docu...
Isolation and Characterization of Parthenogenetic Embryonic Stem (pES) Cells Containing Genetic Background of the Kunming Mouse Strain
Mouse Parthenogenetical Activation Parthenogenetic Embryonic Stem (pES) Cells Kunming Mouse (Mus musculus Km) Genetic Background
2016/5/5
Parthenogenetic embryonic stem (pES) cells could provide a valuable model for research into genomic imprinting and X-linked diseases. In this study, pES cell lines were established from oocytes of hyb...
小鼠canstatin及其N端片段在大肠杆菌BL21中的表达Expression of Mouse canstatin and its N-domain in E.coli BL21
canstatin 血管生成抑制素 RT-PCR cDNA克隆
2008/1/20
摘要 以小鼠肝脏组织总RNA为模板，通过RT-PCR扩增小鼠canstatin及其N端片段基因，克隆到pMD18-T载体中并进行序列分析。将小鼠canstatin及其N端片段基因定向克隆于原核表达载体pET30a(+)中,分别构建表达质粒pET/Can和pET/Can-N, 转化大肠杆菌BL21(DE3), IPTG诱导表达。结果表明: 小鼠canstatin的cDNA长度为684bp，编码227...
筛选代表小鼠植入前胚胎紧密化相关基因的ESTIdentification of ESTs of Genes Related to Compaction in Preimplantation Embryos of Mouse
植入前 紧密化 抑制消减杂交 差异表达 小鼠
2008/1/10
摘要
分别收集181及241枚昆明白小鼠8细胞早期胚胎及8细胞紧密化胚胎，采用SMART PCR方法直接合成胚胎双链cDNA。进而运用抑制消减杂交技术（SSH）对8细胞早期胚胎及8细胞紧密化胚胎的基因表达进行研究，并将所获得的差异表达产物按片段大小分段分离纯化后克隆入pUCm-T载体中，经PCR鉴定后挑选阳性克隆进行测序，筛选出27个代表8细胞早期胚胎和紧密化8细胞胚胎差别表达基因的cDNA片段...
基因敲除是研究生物体基因功能的有效手段。通过基因敲除建立的鼠疾病模型，在研究基因功能及人类疑难病症致病机制等方面发挥着前所未有的作用。本文对目前已获得的基因敲除鼠疾病模型进行了分类和总结，为相关研究的展开奠定了基础。