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Modeling Spatial Correlation of DNA Deformation: DNA Allostery in Protein Binding
Modeling Spatial Correlation DNA Deformation DNA Allostery Protein Binding
2014/9/24
We report a study of DNA deformations using a coarsegrained mechanical model and quantitatively interpret the allosteric effects in protein−DNA binding affinity. A recent single-molecule study (...
Cellular distribution of activity for three enzymes with maltose binding protein as fusion partner and the structural implications
MBP fusion protein periplasmic secretion protein length secondary structure folding rate
2010/10/14
The bacterial SEC pathway is commonly used for secretion of heterologous proteins in E. coli by fusing them to transported proteins to facilitate downstream processing. While some proteins are translo...
Production of an E. coli Toxin Protein; Colicin A in E. coli Using an Inducible System
Protein production toxin colicins immunity protein
2010/4/21
Colicins are bacterial toxins that kill Escherichia coli and related cells; their mode of action is of interest in protein import and toxicology. Colicins translocate across the E. coli outer membrane...