搜索结果: 1-5 共查到“动物遗传学 E. coli”相关记录5条 . 查询时间(0.062 秒)
Identification and Screening of Gene(s) Related to Susceptibility to Enterotoxigenic Escherichia coli F4ab/ac in Piglets
ETEC F4ab/ac Susceptibility Receptor Muc4 Ep-CAM Piglets
2016/4/27
In 2004, J첩rgensen and coworkers proposed the MUC4 gene as a candidate gene of enterotoxigenic Escherichia coli (ETEC) F4ab/ac receptor in piglets and a mutation of G??C in intron 7 of MUC4 was...
Escherichia coli gal K和gpt基因注人小鼠受精卵的研究
显微注射 杂交 凝胶电泳
2008/1/21
摘要含有E. coli gaIK和gpt基因的重组DNA pPB22用显微注射法导入昆明白小鼠受精
卵，经发育，最后得到21只成年小鼠。其中1只小鼠的肝脏显示出E. coli gpt酶活性，但未检测出E. coli galK酶活性。印迹分子杂交表明，外源的pPB 22重组DNA已整合到2只小鼠的染色体上，其中包括那只肝脏中显示E. cola gpt酶活性的小鼠。
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小鼠canstatin及其N端片段在大肠杆菌BL21中的表达Expression of Mouse canstatin and its N-domain in E.coli BL21
canstatin 血管生成抑制素 RT-PCR cDNA克隆
2008/1/20
摘要 以小鼠肝脏组织总RNA为模板，通过RT-PCR扩增小鼠canstatin及其N端片段基因，克隆到pMD18-T载体中并进行序列分析。将小鼠canstatin及其N端片段基因定向克隆于原核表达载体pET30a(+)中,分别构建表达质粒pET/Can和pET/Can-N, 转化大肠杆菌BL21(DE3), IPTG诱导表达。结果表明: 小鼠canstatin的cDNA长度为684bp，编码227...
β-Galactosidase Gene of Thermus thermophilus KNOUC112 Isolated from Hot Springs of a Volcanic Area in New Zealand: Identification of the Bacteria, Cloning and Expression of the Gene in Escherichia coli
Thermus thermophilus Cloning and Expression
2016/4/18
To isolate the β-galactosidase producing thermophilic bacteria, samples of mud and water were collected from hot springs of avolcanic area near Golden Springs in New Zealand. Among eleven isolated str...
Production of Leptin in E. coli and Its Effect on Glucose and Acetate Transport and Expression of Uncoupling Protein-2 Gene in Adipose Tissues of Korean Cattle (Hanwoo)
Bovine Leptin 3T3-L1 Cells Adipose Tissues Glucose Transport Acetate Transport Uncoupling Protein-2
2016/4/15
Leptin has a major role in the regulation of food intake and energy homeostasis. In addition, leptin participates in many physiological functions including regulation of lipid metabolism. Bovine recom...