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一种从大豆胞囊线虫中快速提取DNA的方法
大豆 大豆胞囊线虫 DNA提取 生理小种
2016/12/16
由于大豆胞囊线虫（soybean cyst nematode，SCN）特殊的生长环境，用细菌专用DNA试剂盒提取方法提取DNA的质量不理想，而且耗时长，本文介绍了一种快速提取线虫DNA的方法。通过比较细菌专用DNA试剂盒提取法、质粒试剂盒提取法和CTAB法，发现利用质粒提取试剂盒，改良提取步骤，可以快速的提取质量较好的线虫DNA，rDNA-ITS区域序列扩增可有效扩增出目的基因，该方法可以直接用于...
用浸胚法将外源DNA导入水稻的研究进展
水稻 外源DNA 浸胚法 研究进展
2009/4/27
系统地概述了将外源DNA采用浸胚法导入水稻的理论基础、原理、转导机理，浸胚处理及后代分之验证方法；总结了1989以
来我国育种工作者采用该方法将外源DNA导入水稻所选用的供体、受体及所创建的水稻新种质类型；报道了笔者用浸胚法将空心
莲子草DNA导入水稻增强其耐旱性和创建水稻新种质的研究进展。
Effect of Lyophilization of Cotton Tissue on Quality of Extractable DNA, RNA, and Protein
Extractable DNA Cotton Tissue Lyophilization
2008/6/4
Molecular genetic analysis in cotton (Gossypium hirsutum L.) is often limited by the availability of fresh tissue and the time necessary to extract deoxyribonucleic acid (DNA), ribonucleic acid (RNA),...
玉米S型CMS为配子体雄性不育类型，其线粒体DNA上的R区被证明与胞质育性密切相关。R区含有orf355和orf77两个开放阅读框，被认为是玉米S-CMS重要的胞质育性候选基因。本研究通过对一组同质异核的S型CMS材料R区转录产物的Northern分析和RT-PCR检测，揭示了线粒体DNA中R区的双链转录模式及其中orf355-orf77 mRNA链转录长度的组织特异性，并发现核基因rf3和Rf3...
增强UV-B辐射对作物生理代谢、DNA和蛋白质的影响研究进展
2007/8/7
大气平流层的臭氧层逐渐破坏导致太阳辐射中抵达地球表面的UV-B辐射增加，对作物产生不同程度的影响.本文讨论了UV-B辐射增强对作物生理代谢、DNA损伤和蛋白质含量的影响.UV-B辐射增强，作物叶片类黄酮含量增加、叶绿素含量降低、光合作用减弱，同时UV-B辐射诱致基因活性变化,导致DNA损伤和蛋白质含量的改变.
Use of DNA Markers in Breeding for Bunt Resistance at the National Agricultural Research and Development Institute Fundulea, Romania
wheat breeding molecular markers DNA polymorphism Bt genes
2014/3/13
Breeding high-yielding bunt resistant wheat cultivars faces many difficulties related to: (1) The poor
agronomic performance of most known sources of resistance. Our data show that, even after sever...
Diagnostic of Wheat Leaf Rust Resistance Genes by DNA Markers and their Application in MAS
Lr genes PCR wheat Puccinia triticina MAS
2014/3/19
Leaf rust caused by Puccinia triticina belongs to the most important wheat diseases in Europe, including
Slovakia. Winter wheat varieties registered in Slovakia have been tested with already develop...