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cDNA Microarray Analysis of Differential Gene Expression in Boar Testes during the Prepubertal Period
Boar Testis DNA Chip Analysis Gene Expression DNA Sequence
2016/4/29
In an attempt to understand the biochemical mechanism for the synthesis of the anabolic steroid, 19-nortestosterone, produced by prepubertal boar testes and its physiological role, normalized compleme...
摘要为了优化筛检cDNA微阵列中靶基因的最适长度、浓度及点样溶液的种类,设计持家基因beta actin和GAPDH RT-PCR 3对引物,产物长度在189~1 078 bp之间,以乙肝病毒DNA片段为阴性对照,扩增纯化后分别溶于3×SSC、50%DMSO及0.5mol/L碳酸盐缓冲液(pH=9.0)中,调整浓度分别为0.5μg/μL、1.0μg/μL和1.5μg/μL,比较上述不同条件的杂交结...
cDNA Microarray Analysis of the Gene Expression Profile of Swine Muscle
Muscle Genes Swine Expression Profiles cDNA Microarray
2016/4/19
By screening specific genes related to the muscle growth of swine using cDNA microarray technology, a total of 5 novel genes (GF (growth factor) I, II, III, IV and V) were identified. Results of south...
Molecular Cloning of Adipose Tissue-Specific Genes by cDNA Microarray
Microarray Genefilter Adipose Tissue-specific 3T3-L1 Differentiation
2016/4/14
In an attempt to isolate novel molecules that may play a regulatory role in adipocyte differentiation, were devised an experimental strategy to identify adipose tissue-specific genes by modifying cDNA...
利用抑制消减杂交(suppression subtractive hybridization, SSH)和cDNA Microarray筛选肺癌组织、肺癌旁组织和其它肿瘤组织中相互差异表达的基因.方法 将利用SSH构建的BEP2D细胞永生化阶段、恶性转化前阶段和恶性转化阶段三个cDNA文库中的克隆制作在一张芯片上,筛选了15例肺癌组织、5例肺癌旁组织和其他癌组织24例(肝癌、胃癌、食管癌、乳腺癌、...