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A dATP conversion E. coli strain could be constructed when both pyk and adk1 gene were expressed successfully. pyk gene encodes pyruvate kinase (PK) could be expressed, when inserted it before adk1 ge...
Confocal microscopy is frequently used to examine the subcellular distributions of proteins or otherbiological macromolecules, but essentially all interpretation of the patterns in the resulting image...
Automated Interpretation of Protein Subcellular Location Patterns: Implications for Early Cancer Detection and Assessment.
Background: Detailed knowledge of the subcellular location of each expressed protein is critical to a fullunderstanding of its function. Fluorescence microscopy, in combination with methods for fluore...
Fluorescence microscopy is widely used to analyze the distribution of proteins within cells. As currently practiced, the assignment of a protein to a particular organelle is done by visual inspecti...
The ongoing biotechnology revolution promises a complete understanding of the mechanisms by whichcells and tissues carry out their functions. Central to that goal is the determination of the function ...
There is extensive interest in automating the collection, organization and summarization of biological data. Data in the form of figures and accompanying captions in literature present special chal...
The central goal of proteomics is to clarify the mechanism by which each protein in a given cell type carries out itsfunction. Automated protein subcellular location determination by fluorescence micr...
The overall object of proteomics is to characterize all of the proteins expressed in a given cell type. With the rapiddevelopment of random gene tagging technology and high resolution fluorescence mic...
The ongoing biotechnology revolution promises a complete understanding of the mechanisms by which cells and tissues carry out their functions. Central to that goal is the determination of the functi...
We describe the Protein Subcellular Location Image Database (PSLID), which collects and structures 2-D through 5-D fluorescence microscope images, annotations, and derived features in a relational ...
There is extensive interest in automating the collection, organization and analysis of biological data. Data in the form of images present special challenges for such efforts. Since fluorescence mi...
Determination of the functions of all expressed proteins represents one of the major upcoming challenges in computational molecular biology. Since subcellular location plays a crucial role in prote...
This paper describes a calibration method for determining the physical location of the ultrasound (US) image plane relative to a rigidly attached 3D position sensor. A calibrated US probe can measure ...
In this paper, we propose a method for estimating both the territory and location of single motor units (MUs) of the first dorsal interosseous muscle (FDI) by comparing the observed surface motor unit...

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